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Sylvain Guérin

Professeur titulaire

Sylvain Guérin
Centre de recherche en organogenèse expérimentale
Centre de recherche du CHU de Québec - Université Laval
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Contribution à la recherche

Axe de recherche de l'Université Laval :

L’humain dans son environnement

Thématiques de recherche de la Faculté de médecine :

Médecine régénératrice et moléculaire
Oncologie
Reproduction, génétique, périnatalité et développement

Domaines et intérêts de recherche du (de la) professeur(e) :

Cancer
  • Signalisation cellulaire et cancer
  • Gène suppresseur
  • Génétique du cancer
Neurosciences, santé mentale et toxicomanies
  • Maladies de l'oeil et du système visuel

Projets de recherche

  • Un nouveau vecteur de médicaments ophtalmiques à base de nanoparticules d'or - Instituts de recherche en santé du Canada - Subvention Projet, co-chercheur - 2020-10-01 au 2025-09-30
  • Fonds institutionnel d'exploitation des infrastructures pour l'Université Laval - Fondation Canadienne pour l'innovation (La) - Fonds d'exploitation des infrastructures (FEI), co-chercheur - 2002-04-01 au 2025-03-31
  • Développement d’un outil de detection des mucines oculaires pour l’amélioration de la médication en ophtalmologie - Fonds de recherche du Québec - Santé - Réseaux thématiques de recherche, co-chercheur - 2022-02-01 au 2023-03-31
  • Récepteur 2B de la sérotonine (HTR2B) : mode de régulation et contribution aux propriétés métastatiques du mélanome uvéal - Société de recherche sur le cancer - Subvention de fonctionnement, chercheur principal - 2019-09-01 au 2022-08-31
  • Développement d'une plateforme moléculaire de dosage des mucines oculaires pour l'amélioration de la médication en ophtalmologie - Fonds de recherche du Québec - Nature et technologies - Programme Samuel-De Champlain, co-chercheur - 2019-04-01 au 2022-05-31
  • Les gènes de la famille NFI: patron d'expression et mode de régulation - Conseil de recherches en sciences naturelles et génie Canada, Secrétariat Inter-Conseils (Canada) (CRSH, CRSNG, IRSC) - Subventions à la découverte SD (individuelles et d'équipe), chercheur principal - 2017-04-01 au 2022-03-31
  • Monitoring expression of the transcription factors Sp1 and NFI as markers of cell quality in human corneal epithelial cells cultured on different cell carriers - Fonds de recherche du Québec - Santé - Réseaux thématiques de recherche, chercheur principal - 2021-02-01 au 2022-03-31
  • Impact des exosomes cornéens sur la guérison des plaies de cornées humaines : étude in vitro dans un modèle reconstruit par génie tissulaire - Fonds de recherche du Québec - Santé - Réseaux thématiques de recherche, chercheur principal - 2020-04-01 au 2021-03-31
  • Financement d'un projet de recherche en médecine régénératrice - Fondation du CHU de Québec, chercheur principal - 2020-03-02 au 2021-03-31

Publications

  • The WNK1 kinase regulates the stability of transcription factors during wound healing of human corneal epithelial cells, Pascale Desjardins, Gaëtan Le‐Bel, Sergio C. Ghio, Lucie Germain, Sylvain L. Guérin, Journal of Cellular Physiology, 2022, 10.1002/jcp.30698
  • Contribution of the STAT Family of Transcription Factors to the Expression of the Serotonin 2B (HTR2B) Receptor in Human Uveal Melanoma, Manel Benhassine, Gaëtan Le-Bel, Sylvain L. Guérin, International Journal of Molecular Sciences, 2022, 10.3390/ijms23031564
  • Moyamoya Disease Susceptibility Gene RNF213 Regulates Endothelial Barrier Function., , Stroke, 2022, 10.1161/strokeaha.120.032691
  • Contribution of the Transcription Factors Sp1/Sp3 and AP-1 to Clusterin Gene Expression during Corneal Wound Healing of Tissue-Engineered Human Corneas. , , International journal of molecular sciences, 2021, 10.3390/ijms222212426
  • Investigation of Omega-3 Polyunsaturated Fatty Acid Biological Activity in a Tissue-Engineered Skin Model Involving Psoriatic Cells, Mélissa Simard, Geneviève Rioux, Sophie Morin, Cyril Martin, Sylvain L. Guérin, Nicolas Flamand, Pierre Julien, Julie Fradette, Roxane Pouliot, Journal of Investigative Dermatology, 2021, 10.1016/j.jid.2021.02.755
  • Development of a 3D psoriatic skin model optimized for infiltration of IL-17A producing T cells: Focus on the crosstalk between T cells and psoriatic keratinocytes., , Acta biomaterialia, 2021, 10.1016/j.actbio.2021.09.018
  • The Human Tissue-Engineered Cornea (hTEC): Recent Progress. , , International journal of molecular sciences, 2021, 10.3390/ijms22031291
  • Transcriptome Profiling Analyses in Psoriasis: A Dynamic Contribution of Keratinocytes to the Pathogenesis. , , Genes, 2020, 10.3390/genes11101155
  • The Self-assembly Approach as a Tool for the Tissue Engineering of a Bi-lamellar Human Cornea., , Methods in molecular biology (Clifton, N.J.), 2020, 10.1007/978-1-0716-0599-8_8
  • Irradiated Human Fibroblasts as a Substitute Feeder Layer to Irradiated Mouse 3T3 for the Culture of Human Corneal Epithelial Cells: Impact on the Stability of the Transcription Factors Sp1 and NFI. , , International journal of molecular sciences, 2019, 10.3390/ijms20246296
  • Contribution of the WNK1 kinase to corneal wound healing using the tissue‐engineered human cornea as an in vitro model, Pascale Desjardins, Camille Couture, Lucie Germain, Sylvain L. Guérin, Journal of Tissue Engineering and Regenerative Medicine, 2019, 10.1002/term.2912
  • Grafting of an autologous tissue-engineered human corneal epithelium to a patient with limbal stem cell deficiency (LSCD)., , American journal of ophthalmology case reports, 2019, 10.1016/j.ajoc.2019.100532
  • Analysis of the proteasome activity and the turnover of the serotonin receptor 2B (HTR2B) in human uveal melanoma., , Experimental eye research, 2019, 10.1016/j.exer.2019.04.013
  • Qualitatively Monitoring Binding and Expression of the Transcription Factors Sp1 and NFI as a Useful Tool to Evaluate the Quality of Primary Cultured Epithelial Stem Cells in Tissue Reconstruction., , Methods in molecular biology (Clifton, N.J.), 2019, 10.1007/7651_2018_153
  • Transcription of the Human 5-Hydroxytryptamine Receptor 2B (HTR2B) Gene Is under the Regulatory Influence of the Transcription Factors NFI and RUNX1 in Human Uveal Melanoma. , , International journal of molecular sciences, 2018, 10.3390/ijms19103272
  • The Tissue-Engineered Human Psoriatic Skin Substitute: A Valuable In Vitro Model to Identify Genes with Altered Expression in Lesional Psoriasis. , , International journal of molecular sciences, 2018, 10.3390/ijms19102923
  • The presence of a feeder layer improves human corneal endothelial cell proliferation by altering the expression of the transcription factors Sp1 and NFI., , Experimental eye research, 2018, 10.1016/j.exer.2018.07.009
  • Enhanced wound healing of tissue-engineered human corneas through altered phosphorylation of the CREB and AKT signal transduction pathways., , Acta biomaterialia, 2018, 10.1016/j.actbio.2018.04.021
  • Effects of Long-term Serial Passaging on the Characteristics and Properties of Cell Lines Derived From Uveal Melanoma Primary Tumors., , Investigative ophthalmology & visual science, 2016, 10.1167/iovs.16-19317
  • Tissue-engineered human psoriatic skin supplemented with cytokines as an in vitro model to study plaque psoriasis., , Regenerative medicine, 2016, 10.2217/rme-2016-0037
  • A Role for DLK in Microtubule Reorganization to the Cell Periphery and in the Maintenance of Desmosomal and Tight Junction Integrity., , The Journal of investigative dermatology, 2016, 10.1016/j.jid.2016.07.035
  • The tissue-engineered human cornea as a model to study expression of matrix metalloproteinases during corneal wound healing., , Biomaterials, 2015, 10.1016/j.biomaterials.2015.11.006
  • Functional Impact of Collagens on the Activity Directed by the Promoter of the α5 Integrin Subunit Gene in Corneal Epithelial Cells., , Investigative ophthalmology & visual science, 2015, 10.1167/iovs.15-16587
  • Characterization of the human α9 integrin subunit gene: Promoter analysis and transcriptional regulation in ocular cells., , Experimental eye research, 2015, 10.1016/j.exer.2015.03.001
  • Contribution of Sp1 to Telomerase Expression and Activity in Skin Keratinocytes Cultured With a Feeder Layer., , Journal of cellular physiology, 2015, 10.1002/jcp.24706
  • Expression of the α5 integrin gene in corneal epithelial cells cultured on tissue-engineered human extracellular matrices., , Biomaterials, 2013, 10.1016/j.biomaterials.2013.05.007
  • Irradiated human dermal fibroblasts are as efficient as mouse fibroblasts as a feeder layer to improve human epidermal cell culture lifespan., , International journal of molecular sciences, 2013, 10.3390/ijms14034684
  • A tissue-engineered corneal wound healing model for the characterization of reepithelialization., , Methods in molecular biology (Clifton, N.J.), 2013, 10.1007/978-1-62703-505-7_4
  • Qualitatively monitoring binding and expression of the transcription factor Sp1 as a useful tool to evaluate the reliability of primary cultured epithelial stem cells in tissue reconstruction., , Methods in molecular biology (Clifton, N.J.), 2013, 10.1007/978-1-62703-330-5_11
  • Rescue of the transcription factors Sp1 and NFI in human skin keratinocytes through a feeder-layer-dependent suppression of the proteasome activity., , Journal of molecular biology, 2012, 10.1016/j.jmb.2012.01.021
  • Suppression of α5 gene expression is closely related to the tumorigenic properties of uveal melanoma cell lines., , Pigment cell & melanoma research, 2011, 10.1111/j.1755-148x.2011.00869.x
  • Identification of differentially expressed genes in uveal melanoma using suppressive subtractive hybridization., , Molecular vision, 2011
  • Reconstruction of a human cornea by the self-assembly approach of tissue engineering using the three native cell types., , Molecular vision, 2010
  • Tissue engineering of skin and cornea: Development of new models for in vitro studies., , Annals of the New York Academy of Sciences, 2010, 10.1111/j.1749-6632.2009.05373.x
  • Impact of cell source on human cornea reconstructed by tissue engineering., , Investigative ophthalmology & visual science, 2009, 10.1167/iovs.08-2001
  • Electrophoretic mobility shift assays for the analysis of DNA-protein interactions., , Methods in molecular biology (Clifton, N.J.), 2009, 10.1007/978-1-60327-015-1_2
  • Differential binding of the transcription factors Sp1, AP-1, and NFI to the promoter of the human alpha5 integrin gene dictates its transcriptional activity., , Investigative ophthalmology & visual science, 2008, 10.1167/iovs.08-2059
  • Transcriptional regulation of the human alpha6 integrin gene by the transcription factor NFI during corneal wound healing., , Investigative ophthalmology & visual science, 2008, 10.1167/iovs.08-1913
  • Characterization of wound reepithelialization using a new human tissue-engineered corneal wound healing model., , Investigative ophthalmology & visual science, 2008, 10.1167/iovs.07-0904
  • Human NCU-G1 can function as a transcription factor and as a nuclear receptor co-activator., , BMC molecular biology, 2007, 10.1186/1471-2199-8-106
  • Regulation of poly(ADP-ribose) polymerase-1 (PARP-1) gene expression through the post-translational modification of Sp1: a nuclear target protein of PARP-1., , BMC molecular biology, 2007, 10.1186/1471-2199-8-96
  • Laminin reduces expression of the human alpha6 integrin subunit gene by altering the level of the transcription factors Sp1 and Sp3., , Investigative ophthalmology & visual science, 2007, 10.1167/iovs.07-0016
  • Control of integrin genes expression in the eye., , Progress in retinal and eye research, 2006, 10.1016/j.preteyeres.2006.10.004
  • Transcriptional regulation of the cyclin-dependent kinase inhibitor 1A (p21) gene by NFI in proliferating human cells., , Nucleic acids research, 2006, 10.1093/nar/gkl861
  • Expression of the gene encoding poly(ADP-ribose) polymerase-1 is modulated by fibronectin during corneal wound healing., , Investigative ophthalmology & visual science, 2006, 10.1167/iovs.06-0176
  • The feeder layer-mediated extended lifetime of cultured human skin keratinocytes is associated with altered levels of the transcription factors Sp1 and Sp3., , Journal of cellular physiology, 2006, 10.1002/jcp.20529
  • Autologous transplantation of rabbit limbal epithelia cultured on fibrin gels for ocular surface reconstruction., , Molecular vision, 2006
  • MMP-2 expression in uveal melanoma: differential activation status dictated by the cellular environment., , Molecular vision, 2005
  • Regulation of gene expression: probing DNA-protein interactions in vivo and in vitro., , Expert review of proteomics, 2005, 10.1586/14789450.2.5.705
  • SW480, a p53 double-mutant cell line retains proficiency for some p53 functions., , Journal of molecular biology, 2005, 10.1016/j.jmb.2005.06.033
  • Regulation of the poly(ADP-ribose) polymerase-1 gene expression by the transcription factors Sp1 and Sp3 is under the influence of cell density in primary cultured cells., , The Biochemical journal, 2005, 10.1042/bj20041718
  • Role of the extracellular matrix proteins in the resistance of SP6.5 uveal melanoma cells toward cisplatin., , International journal of oncology, 2005
  • Expression of phospholipases A2 and C in human corneal epithelial cells., , Investigative ophthalmology & visual science, 2004, 10.1167/iovs.04-0084
  • Expression of glial fibrillary acidic protein in primary cultures of human Müller cells., , Experimental eye research, 2004, 10.1016/j.exer.2004.05.008
  • Integrin alpha5 expression by the ARPE-19 cell line: comparison with primary RPE cultures and effect of growth medium on the alpha5 gene promoter strength., , Experimental eye research, 2004, 10.1016/j.exer.2004.03.004
  • A conserved initiator element on the mammalian poly(ADP-ribose) polymerase-1 promoters, in combination with flanking core elements, is necessary to obtain high transcriptional activity., , Biochimica et biophysica acta, 2004, 10.1016/j.bbaexp.2004.04.003
  • Expression of the alpha4 integrin subunit gene promoter is modulated by the transcription factor Pax-6 in corneal epithelial cells., , Investigative ophthalmology & visual science, 2004, 10.1167/iovs.03-0908
  • Effect of quiescence on integrin alpha5beta1 expression in human retinal pigment epithelium., , Molecular vision, 2003
  • Regulation of the integrin subunit alpha5 gene promoter by the transcription factors Sp1/Sp3 is influenced by the cell density in rabbit corneal epithelial cells., , Investigative ophthalmology & visual science, 2003, 10.1167/iovs.03-0191
  • Influence of sp1/sp3 expression on corneal epithelial cells proliferation and differentiation properties in reconstructed tissues., , Investigative ophthalmology & visual science, 2003, 10.1167/iovs.02-0707
  • Members of the nuclear factor 1 family reduce the transcriptional potential of the nuclear receptor LXRalpha promoter., , Biochemical and biophysical research communications, 2001, 10.1006/bbrc.2001.6078
  • Tumorigenicity of the mixed spindle-epithelioid SP6.5 and epithelioid TP17 uveal melanoma cell lines is differentially related to alpha5beta1 integrin expression., , Investigative ophthalmology & visual science, 2001
  • Nuclear factor 1 interferes with Sp1 binding through a composite element on the rat poly(ADP-ribose) polymerase promoter to modulate its activity in vitro., , The Journal of biological chemistry, 2001, 10.1074/jbc.m010360200
  • Electrophoretic mobility shift assays for the analysis of DNA-protein interactions., , Methods in molecular biology (Clifton, N.J.), 2001, 10.1385/1-59259-208-2:013
  • Expression of the alpha 5 integrin subunit gene promoter is positively regulated by the extracellular matrix component fibronectin through the transcription factor Sp1 in corneal epithelial cells in vitro., , The Journal of biological chemistry, 2000, 10.1074/jbc.m002945200
  • Can we produce a human corneal equivalent by tissue engineering?, , Progress in retinal and eye research, 2000, 10.1016/s1350-9462(00)00005-7
  • Expression of integrin alpha5beta1 and MMPs associated with epithelioid morphology and malignancy of uveal melanoma., , Investigative ophthalmology & visual science, 2000
  • Reconstructed human cornea produced in vitro by tissue engineering., , Pathobiology : journal of immunopathology, molecular and cellular biology, 1999, 10.1159/000028064
  • Multiple cloning sites from mammalian expression vectors interfere with gene promoter studies in vitro., , European journal of biochemistry, 1999, 10.1046/j.1432-1327.1999.00362.x
  • Transcriptional regulation of the alpha 4 integrin subunit gene in the metastatic spread of uveal melanoma., , Anticancer research, 1998
  • Improving sensitivity of the electrophoretic mobility shift assay by restricting tissue phosphatase activities., , BioTechniques, 1998, 10.2144/98246st02
  • Transcriptional regulation of the rat poly(ADP-ribose) polymerase gene by Sp1., , European journal of biochemistry, 1997, 10.1111/j.1432-1033.1997.0342a.x
  • The rat growth hormone and human cellular retinol binding protein 1 genes share homologous NF1-like binding sites that exert either positive or negative influences on gene expression in vitro., , DNA and cell biology, 1997, 10.1089/dna.1997.16.951
  • A nuclear factor other than Sp1 binds the GC-rich promoter of the gene encoding rat poly(ADP-ribose) polymerase in vitro., , Biochemistry and cell biology = Biochimie et biologie cellulaire, 1997, 10.1139/bcb-75-4-427
  • Multiple nuclear regulatory proteins bind a single cis-acting promoter element to control basal transcription of the human alpha 4 integrin gene in corneal epithelial cells., , DNA and cell biology, 1996, 10.1089/dna.1996.15.779
  • Optimization of competitor poly(dI-dC).poly(dI-dC) levels is advised in DNA-protein interaction studies involving enriched nuclear proteins., , BioTechniques, 1996, 10.2144/19962003439
  • Overlapping cis-acting elements located in the first intron of the gene for type I 3 beta-hydroxysteroid dehydrogenase modulate its transcriptional activity., , Molecular endocrinology (Baltimore, Md.), 1995, 10.1210/mend.9.11.8584035
  • Multiple regulatory elements control the basal promoter activity of the human alpha 4 integrin gene., , DNA and cell biology, 1994, 10.1089/dna.1994.13.1071
  • The rat growth hormone proximal silencer contains a novel DNA-binding site for multiple nuclear proteins that represses basal promoter activity., , European journal of biochemistry, 1994, 10.1111/j.1432-1033.1994.00419.x
  • Binding of a member of the NF1 family of transcription factors to two distinct cis-acting elements in the promoter and 5'-flanking region of the human cellular retinol binding protein 1 gene., , Molecular endocrinology (Baltimore, Md.), 1994, 10.1210/mend.8.6.7935489
  • The 30-kDa rat liver transcription factor nuclear factor 1 binds the rat growth-hormone proximal silencer., , European journal of biochemistry, 1994, 10.1111/j.1432-1033.1994.tb18560.x
  • The US-1 element from the gene encoding rat poly(ADP-ribose) polymerase binds the transcription factor Sp1., , European journal of biochemistry, 1993, 10.1111/j.1432-1033.1993.tb18008.x
  • A simple apparatus for fast and inexpensive recovery of DNA from polyacrylamide gels., , BioTechniques, 1993
  • Two distinct nuclear proteins bind to the rat growth hormone silencer-1 element., , Annals of the New York Academy of Sciences, 1993, 10.1111/j.1749-6632.1993.tb32286.x
  • Expression of the rat growth-hormone gene is under the influence of a cell-type-specific silencer element., , European journal of biochemistry, 1993, 10.1111/j.1432-1033.1993.tb17774.x
  • Salt-dependent formation of DNA/protein complexes in vitro, as viewed by the gel mobility shift assay., , BioTechniques, 1992
  • Transcription of the mouse secretory protease inhibitor p12 gene is activated by the developmentally regulated positive transcription factor Sp1., , Molecular and cellular biology, 1992, 10.1128/mcb.12.9.3796-3806.1992
  • Demonstration of DNA binding factors interacting with a fragment of the canine prostate arginine esterase gene promoter., , FEBS letters, 1992, 10.1016/0014-5793(92)80501-7
  • Binding of a nuclear protein to the rat growth hormone silencer element., , Nucleic acids research, 1992, 10.1093/nar/20.3.401
  • An improved CAT assay for promoter analysis in either transgenic mice or tissue culture cells., , DNA and cell biology, 1992, 10.1089/dna.1992.11.83
  • A short protocol for micro-purification of nuclear proteins from whole animal tissue., , BioTechniques, 1991
  • Identification of a DNA-binding site for the transcription factor GC2 in the promoter region of the p12 gene and repression of its positive activity by upstream negative regulatory elements., , The Journal of biological chemistry, 1990, 10.1016/s0021-9258(18)45844-9
  • Characterization of human 3 beta-hydroxysteroid dehydrogenase/delta 5-delta 4-isomerase gene and its expression in mammalian cells., , The Journal of biological chemistry, 1990, 10.1016/s0021-9258(17)30528-8
  • DNAse I footprint analysis of nuclear proteins from pituitary and nonpituitary cells that specifically bind to the rat growth hormone promoter and 5'-regulatory region., , Molecular endocrinology (Baltimore, Md.), 1988, 10.1210/mend-2-11-1101
  • Two mechanisms for the extinction of gene expression in hybrid cells., , Science (New York, N.Y.), 1988, 10.1126/science.2842865
  • Heterogeneity in ampR-ampC gene interaction in Enterobacter cloacae., , Reviews of infectious diseases, 1988, 10.1093/clinids/10.4.786
  • Cloning and characterization of chromosomally encoded cephalosporinase gene of Enterobacter cloacae., , Canadian journal of microbiology, 1986, 10.1139/m86-061
  • Expression of class I genes in the major histocompatibility complex: identification of eight distinct mRNAs in DBA/2 mouse liver., , Cell, 1985, 10.1016/s0092-8674(85)80020-9

Contribution à l'enseignement aux cycles supérieurs

Étudiant(e)s dirigé(e)s*

Depuis 2009
  • Pascale Desjardins - Doctorat - En cours
  • Geneviève Rioux - Doctorat - En cours
  • Christelle Gross - Post-doctorat - En cours
  • Vanessa Molloy-Simard - Maitrîse avec mémoire - 2011/05
  • Marjorie-Allison Bergeron - Maitrîse avec mémoire - 2013/01
  • Camille Couture - Maitrîse avec mémoire - 2016/05
  • Gaëtan Le Bel - Doctorat - 2020/05

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*Les supervisions d’étudiant(e)s de 1er cycle en stage de recherche et de résident(e)s aux études médicales postdoctorales seront répertoriées ultérieurement.

Direction et personnes-ressources

Vice-décanat à la recherche et aux études supérieures
Pavillon Ferdinand-Vandry
Université Laval
1050, avenue de la Médecine, local 4645
Québec (Québec) G1V 0A6
Canada

Renseignements - Secrétariat
418 656-2690
vdres@fmed.ulaval.ca

Jacques Simard
Vice-doyen à la recherche et aux études supérieures
vice-doyen.recherche-et-etudes-superieures@fmed.ulaval.ca

Louise Laperrière
Adjointe au vice-doyen à la recherche et aux études supérieures
louise.laperriere@fmed.ulaval.ca 

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