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Patrick Provost

Professeur titulaire

Patrick Provost
Centre de recherche du CHU de Québec - Université Laval
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Contribution à la recherche

Axe de recherche de l'Université Laval :

Santé et bien-être durables

Thématiques de recherche de la Faculté de médecine :

Médecine régénératrice et médecine moléculaire

Domaines et intérêts de recherche du (de la) professeur(e) :

Génétique humaine
  • Expression et régulation génique
  • Génomique
  • Protéomique
  • Protéines
  • Micropuces d'ADN et d'ARN
  • Maladies génétiques
Nutrition et métabolisme
  • Nutrition
  • Processus de digestion
  • Appareil digestif
  • Pathologies de l'appareil digestif
  • Maladie de Crohn
  • Produits nutraceutiques et aliments fonctionnels
Cellulaire
  • Gène
  • Cellule
Génomique et protéomique

Projets de recherche

  • Fonds institutionnel d'exploitation des infrastructures pour l'Université Laval - Fondation Canadienne pour l'innovation (La) - Fonds d'exploitation des infrastructures (FEI), co-chercheur - 2002-04-01 au 2025-03-31
  • A unique model to assess the oral transfer of dietary microRNAs - Instituts de recherche en santé du Canada - Subvention Projet, chercheur principal - 2019-10-01 au 2024-09-30
  • A novel class of small non-coding RNAs - Secrétariat Inter-Conseils (Canada) (CRSH, CRSNG, IRSC), Conseil de recherches en sciences naturelles et génie Canada - Subventions à la découverte SD (individuelles et d'équipe), chercheur principal - 2019-04-01 au 2024-03-31

Publications

  • Activated platelets can deliver mRNA regulatory Ago2bulletmicroRNA complexes to endothelial cells via microparticles., Provost, Patrick, Boilard, Eric, Cloutier, Nathalie, Duchez, Anne-Claire, Ple, Helene, Corduan, Aurelie, Laffont, Benoit, Blood, 2013, 10.1182/blood-2013-03-492801
  • MicroRNAs in gene regulation: When the smallest governs it all, Provost, P, Plante, P, Perron, MP, Ouellet, DL, Gobeil, LA, Journal of Biomedicine and Biotechnology, 2006, 10.1155/JBB/2006/69616
  • MicroRNAs as Modulators of the Platelet Proteome, , 1911, http://dx.doi.org/10.2174/157016411797247468
  • Interaction of 5-lipoxygenase with cellular proteins, Samuelsson, B, Radmark, O, Provost, P, Proceedings of the National Academy of Sciences of the United States of America, 1999
  • Involvement of Dcr1 in post-transcriptional regulation of gene expression in Schizosaccharomyces pombe, Rohani, M, Provost, P, Plante, P, Ouellette, M, Gobeil, LA, Frontiers in Bioscience, 2008, 10.2741/2835
  • Regulation of host gene expression by HIV-1 TAR microRNAs, Provost, P., Rossi, J. J., Burnett, J. C., Plante, I., Gobeil, L. A., Létourneau, K., Vigneault-Edwards, J., Ouellet, D. L., Retrovirology, 2013, 10.1186/1742-4690-10-86
  • SUSTAINED MYOCARDIAL PROTECTION BY CLENTIAZEM (TA-3090) AFTER A 90-MINUTE CORONARY-OCCLUSION AND 72 HOURS OF REPERFUSION IN DOGS WITH COLLATERAL FLOW, ROUSSEAU, G, PROVOST, P, LATOUR, JG, Journal of Cardiovascular Pharmacology, 1993
  • The antiadhesive and antithrombotic effects of the nitric oxide donor SIN-1 are combined with a decreased vasoconstriction in a porcine model of balloon angioplasty, Tremblay, J, Provost, P, Merhi, Y, Arteriosclerosis Thrombosis and Vascular Biology, 1997
  • Dissociation of SERPINE1 mRNA from the translational repressor proteins Ago2 and TIA-1 upon platelet activation, , Thromb Haemost, 2015, 10.1160/th14-07-0622
  • Effects of pathogen reduction systems on platelet microRNAs, mRNAs, activation, and function, Provost, P., Vamvakas, E. C., Hellstern, P., Boilard, E., Laffont, B., Corduan, A., Landry, P., Meyer, C. U., Hitzler, W. E., Osman, A., Platelets, 2014, 10.3109/09537104.2014.898178
  • Coactosin-like protein functions as a stabilizing chaperone for 5-lipoxygenase: role of tryptophan 102, Steinhilber, D, Schneider, G, Samuelsson, B, Rakonjac, M, Radmark, O, Provost, P, Hofmann, B, Fischer, L, Esser, J, Biochemical Journal, 2010, 10.1042/BJ20090856
  • Dicer cleavage by calpain determines platelet microRNA levels and function in diabetes, , Circ Res, 2015, 10.1161/circresaha.117.305784
  • Platelets, neutrophils, and vasoconstriction after arterial injury by angioplasty in pigs: effects of MK-886, a leukotriene biosynthesis inhibitor, Provost, P, Merhi, Y, Borgeat, P, British Journal of Pharmacology, 1998
  • Coactosin-like protein supports 5-lipoxygenase enzyme activity and up-regulates leukotriene A(4) production, Werz, O, Steinhilbert, D, Samuelsson, B, Rakonjac, M, Radmark, O, Provost, P, Fischer, L, Proceedings of the National Academy of Sciences of the United States of America, 2006, 10.1073/pnas.0605150103
  • A mutation interfering with 5-lipoxygenase domain interaction leads to increased enzyme activity, Rådmark, O., Samuelsson, B., Watanabe, T., Steinhilber, D., Hofmann, B., Rinaldo-Matthis, A., Funk, C. D., Chen, X., Persson, B., Provost, P., Tanabe, M., Rakonjac Ryge, M., Arch Biochem Biophys, 2014, 10.1016/j.abb.2014.01.017
  • 5-Lipoxygenase interacts with coactosin-like protein, Samuelsson, B, Radmark, O, Provost, P, Hammarberg, T, Gerisch, G, Doucet, J, Journal of Biological Chemistry, 2001
  • A polymerase chain reaction-based cloning strategy applicable to functional microRNA studies, St-Gelais, N, Provost, P, Plante, I, Boissonneault, V, Analytical Biochemistry, 2008, 10.1016/j.ab.2008.06.026
  • Alteration of the platelet transcriptome in chronic kidney disease, Rousseau, G., Provost, P., Plé, H., Maltais, M., Madore, F., Corduan, A., Thromb Haemost, 2012, 10.1160/TH12-03-0153
  • Detection of human Dicer and Argonaute 2 catalytic activity, Provost, P., Plante, I., Perron, M. P., Landry, P., Methods Mol Biol, 2011, 10.1007/978-1-61779-046-1_9
  • ENDOTHELIUM-DERIVED NITRIC-OXIDE ATTENUATES NEUTROPHIL ADHESION TO ENDOTHELIUM UNDER ARTERIAL FLOW CONDITIONS, WATERS, D, PROVOST, P, MERHI, Y, LAM, JYT, LACOSTE, L, Arteriosclerosis and Thrombosis, 1994
  • Regulation of human Dicer by the resident ER membrane protein CLIMP-63, Pépin, G., Provost, P., Perron, M. P., Nucleic Acids Res, 2012, 10.1093/nar/gks903
  • DICER1 loss and Alu RNA induce age-related macular degeneration via the NLRP3 inflammasome and MyD88, Yoo, J. W., Tarallo, V., Provost, P., Ponicsan, S. L., Ogura, Y., Núñez, G., Murphy, M. P., Lee, D. K., Kugel, J. F., Kleinman, M. E., Kim, Y., Kerur, N., Kaneko, H., Hirano, Y., Hinton, D. R., Hauswirth, W. W., Goodrich, J. A., Gelfand, B. D., Fowler, B. J., Dunaief, J. L., Dridi, S., Cho, W. G., Chiodo, V. A., Chaudhuri, G., Bogdanovich, S., Baffi, J. Z., Ambati, J., Ambati, B. K., Albuquerque, R. J., Cell, 2012, 10.1016/j.cell.2012.03.036
  • MicroRNAs as a molecular basis for mental retardation, Alzheimer's and prion diseases, Provost, P, Brain Research, 2010, 10.1016/j.brainres.2010.03.069
  • Differential Expression Analysis by RNA-Seq Reveals Perturbations in the Platelet mRNA Transcriptome Triggered by Pathogen Reduction Systems, , PLoS One, 2015, 10.1371/journal.pone.0133070
  • The Repertoire and Features of Human Platelet microRNAs, Provost, P., Plé, H., Landry, P., Gunaratne, P. H., Coarfa, C., Benham, A., PLoS One, 2012, 10.1371/journal.pone.0050746
  • Protein interactions and complexes in human microRNA biogenesis and function, Provost, P, Perron, MP, Frontiers in Bioscience, 2008, 10.2741/2865
  • The nuclear RNase III Drosha initiates microRNA processing, Yim, J, Radmark, O, Provost, P, Lee, Y, Lee, J, Kim, VN, Kim, S, Kim, J, Han, JJ, Choi, H, Ahn, C, Nature, 2003, 10.1038/nature01957
  • Modulation of microRNA Activity by Semi-microRNAs, Provost, P., Plé, H., Plante, I., Landry, P., Gunaratne, P. H., Front Genet, 2012, 10.3389/fgene.2012.00099
  • INCREASE OF NEUTROPHIL ADHESION AND VASOCONSTRICTION WITH PLATELET DEPOSITION AFTER DEEP ARTERIAL INJURY BY ANGIOPLASTY, PROVOST, P, MERHI, Y, LEUNG, TK, LAM, JYT, GUIDOIN, R, American Heart Journal, 1995
  • Importance of platelets in neutrophil adhesion and vasoconstriction after deep carotid arterial injury by angioplasty in pigs, Provost, P, Merhi, Y, Latour, JG, Guidoin, R, Arteriosclerosis Thrombosis and Vascular Biology, 1997
  • Human Dicer C-terminus functions as a 5-lipoxygenase binding domain, Samuelsson, B, Rakonjac, M, Radmark, O, Provost, P, Plante, I, Pepin, G, Ouellet, DL, Hermansson, A, Goulet, I, Doucet, J, Dincbas-Renqvist, V, Biochimica Et Biophysica Acta-Gene Regulatory Mechanisms, 2009, 10.1016/j.bbagrm.2008.10.002
  • Ribonuclease activity and RNA binding of recombinant human Dicer, Samuelsson, B, Radmark, O, Provost, P, Frendewey, D, Doucet, J, Dishart, D, Embo Journal, 2002
  • Dicer-mediated upregulation of BCRP confers tamoxifen resistance in human breast cancer cells, Weiss, H., Tsimelzon, A., Selever, J., Provost, P., Miele, L., Lewis, M. T., Herynk, M. H., Gu, G., Fuqua, S. A., Dontu, G., Di Pietro, A., Covington, K. R., Boumendjel, A., Beyer, A., Barone, I., Ando, S., Albain, K., Clin Cancer Res, 2011, 10.1158/1078-0432.CCR-11-1403
  • Resistance of human hepatitis delta virus RNAs to dicer activity, Taylor, JM, Provost, P, Chang, JH, Journal of Virology, 2003, 10.1128/JVI.77.22.11910-11917.2003
  • Interpretation and applicability of microRNA data to the context of Alzheimer's and age-related diseases, Provost, P, Aging-Us, 2010
  • Coactosin-like protein, a human F-actin-binding protein: critical role lysine-75, Stock, A, Samuelsson, B, Radmark, O, Provost, P, Gerisch, G, Doucet, J, Biochemical Journal, 2001
  • Platelet microparticles are internalized in neutrophils via the concerted activity of 12-lipoxygenase and secreted phospholipase A2-IIA, , Proc Natl Acad Sci U S A, 2015, 10.1073/pnas.1507905112
  • Existence of a microRNA pathway in anucleate platelets, Rousseau, G, Provost, P, Plante, I, Perron, MP, Ouellet, DL, Landry, P, Nature Structural & Molecular Biology, 2009, 10.1038/nsmb.1651
  • DICER1 deficit induces Alu RNA toxicity in age-related macular degeneration, Zhang, Q., Yoo, J. W., Witta, J., Tarallo, V., Song, Y., Rudin, C. M., Provost, P., Provis, J. M., Ponicsan, S. L., Misra, S., Milam, A. H., Madigan, M. C., Littman, D. R., Lee, D. K., Kugel, J. F., Kleinman, M. E., Karikó, K., Kaneko, H., Justice, N. L., Hirano, Y., Hinton, D. R., Hauswirth, W. W., Hadziahmetovic, M., Grossniklaus, H. E., Goodrich, J. A., Gelfand, B. D., Fuchs, E., Fowler, B. J., Dunaief, J. L., Dridi, S., Chong, M. M., Cho, W. G., Chiodo, V. A., Chaudhuri, G., Buaas, F. W., Braun, R. E., Bogdanovich, S., Blandford, A. D., Baffi, J. Z., Ambati, J., Ambati, B. K., Albuquerque, R. J., Nature, 2011, 10.1038/nature09830
  • MicroRNA-298 and MicroRNA-328 Regulate Expression of Mouse beta-Amyloid Precursor Protein-converting Enzyme 1, Rivest, S, Provost, P, Plante, I, Boissonneault, V, Journal of Biological Chemistry, 2009, 10.1074/jbc.M807530200
  • Letter to the Editor: NMR structure of human coactosin-like protein, Samuelsson, B, Rakonjac, M, Radmark, O, Provost, P, Otting, G, Liepinsh, E, Boissonneault, B, Journal of Biomolecular Nmr, 2004
  • Endothelium-derived nitric oxide decreases polymorphonuclear leukocyte interaction with the deeply injured arterial wall under intermediate and high shear conditions, Provost, P, Merhi, Y, Thrombosis and Haemostasis, 1997
  • Selectin blockade reduces neutrophil interaction with platelets at the site of deep arterial injury by angioplasty in pigs, Theoret, JF, Provost, P, Phillips, ML, Merhi, Y, Latour, JG, Chauvet, P, Arteriosclerosis Thrombosis and Vascular Biology, 1999
  • HIV-1 and the microRNA-guided silencing pathway: An intricate and multifaceted encounter, Tremblay, MJ, Provost, P, Barat, C, Plante, I, Virus Research, 2006, 10.1016/j.virusres.2006.06.015
  • Platelet microparticles reprogram macrophage gene expression and function, , Thromb Haemost, 2015, 10.1160/th15-05-0389
  • Protein Components of the microRNA Pathway and Human Diseases, , 1911, http://dx.doi.org/10.1007/978-1-60327-547-7_18
  • BW755C, a dual lipoxygenase cyclooxygenase inhibitor, reduces mural platelet and neutrophil deposition and vasoconstriction after angioplasty injury in pigs, Provost, P, Merhi, Y, Journal of Pharmacology and Experimental Therapeutics, 1996
  • Ago1 and Dcr1, two core components of the RNA interference pathway, functionally diverge from Rdp1 in regulating cell cycle events in Schizosaccharomyces pombe, Provost, P, Hobman, TC, Ekwall, K, Carmichael, JB, Molecular Biology of the Cell, 2004, 10.1091/mbc.E03-06-0433
  • Identification of functional microRNAs released through asymmetrical processing of HIV-1 TAR element, Tremblay, MJ, Provost, P, Plante, I, Ouellet, DL, Landry, P, Janelle, ME, Flamand, L, Barat, C, Nucleic Acids Research, 2008, 10.1093/nar/gkn076
  • Hypothesis: A role for fragile X mental retardation protein in mediating and relieving microRNA-guided translational repression?, Provost, P, Plante, I, Journal of Biomedicine and Biotechnology, 2006, 10.1155/JBB/2006/16806
  • Calcium binding site of 5-lipoxygenase, Samuelsson, B, Reddy, KV, Radmark, O, Provost, P, Persson, B, Paoletti, R, Nicosia, S, Hammarberg, T, Granstrom, E, Folco, GC, Advances in Prostaglandin and Leukotriene Research: Basic Science and New Clinical Applications, 2001
  • Dicer-derived MicroRNAs are utilized by the fragile X mental retardation protein for assembly on target RNAs, Tremblay, S, Provost, P, Plante, I, Ouellet, DL, Khandjian, EW, Gobeil, LA, Davidovic, L, Journal of Biomedicine and Biotechnology, 2006, 10.1155/JBB/2006/64347
  • Dicer is required for chromosome segregation and gene silencing in fission yeast cells, Wright, A, Walfridsson, J, Silverstein, RA, Samuelsson, B, Radmark, O, Provost, P, Kniola, B, Ekwall, K, Djupedal, I, Dishart, D, Proceedings of the National Academy of Sciences of the United States of America, 2002, 10.1073/pnas.212633199
  • Current knowledge of MicroRNAs and noncoding RNAs in virus-infected cells, Provost, P., Ouellet, D. L., Methods Mol Biol, 2010, 10.1007/978-1-60761-588-0_3
  • Endogenous nitric oxide release modulates mural platelet thrombosis and neutrophil-endothelium interactions under low and high shear conditions, Provost, P, Merhi, Y, Thrombosis Research, 1997
  • Molecular cloning and functional characterization of mouse coactosin-like protein, Samuelsson, B, Radmark, O, Provost, P, Doucet, J, Biochemical and Biophysical Research Communications, 2002, 10.1006/bbrc.2001.6236
  • CLENTIAZEM GIVEN AT REPERFUSION IMPROVES SUBENDOCARDIAL REFLOW AND REDUCES MYOCARDIAL INFARCT SIZE IN THE DOG, TRAN, D, ROUSSEAU, G, PROVOST, P, LATOUR, JG, CAILLE, G, Journal of Pharmacology and Experimental Therapeutics, 1994
  • The N-terminal domain of 5-lipoxygenase binds calcium and mediates calcium stimulation of enzyme activity, Radmark, O, Provost, P, Persson, B, Hammarberg, T, Journal of Biological Chemistry, 2000

Contribution à l'enseignement aux cycles supérieurs

Étudiant(e)s dirigé(e)s*

Depuis 2009
  • Sara Guellal - Doctorat - En cours
  • Raphaël Matusiak - Maîtrise avec mémoire - 2014/01
  • Geneviève Pépin - Doctorat - 2015/01
  • Aurélie Corduan - Doctorat - 2015/05
  • Benoit Laffont - Doctorat - 2015/05
  • Marine Lambert - Doctorat - 2021/01
  • Idrissa Diallo - Doctorat - 2022/09

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*Les supervisions d’étudiant(e)s de 1er cycle en stage de recherche et de résident(e)s aux études médicales postdoctorales seront répertoriées ultérieurement.